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dc.contributor.authorMacedo Júnior, Milton Carvalho-
dc.contributor.authorLucia Júnior, Thomaz-
dc.contributor.authorRambo, Gissele-
dc.contributor.authorFerreira Filho, E. B.-
dc.contributor.authorRosa, A. P.-
dc.contributor.authorFabiane, C.-
dc.contributor.authorCabral, M.-
dc.contributor.authorDeschamps, João Carlos-
dc.date.accessioned2010-10-08T13:35:53Z-
dc.date.available2010-10-08T13:35:53Z-
dc.date.issued2010-02-
dc.identifier.citationMACEDO JÚNIOR, Milton et al. In vitro penetration of swine oocytes by homologous spermatozoa: distinct systems for gamete's co-incubation and oocyte's cryopreservation. Animal Reproduction Science (Print), v. 117, p. 295-301, 2010.pt_BR
dc.identifier.issn0378-4320-
dc.identifier.urihttp://hdl.handle.net/123456789/113-
dc.description.abstractIn vitro penetration (IVP) of swine oocytes by homologous spermatozoa was evaluated in two experiments using four boars as semen donors. In experiment 1, the IVP rate and the number of penetrating spermatozoa (PSP) were compared using three co-incubation systems for vitrified oocytes and fresh sperm: (1) 35mL petri dishes in a CO2 incubator, (2) 35mL petri dishes in bags (submarine system) and (3) glass flasks partially submerged in water bath with the same gas mixture used for the bag system. Mean PSPwas 8.2±10.1 and the IVP ratewas 90.5%. The PSP differed across all systems (P = 0.0006): 15.5±0.5 for flasks, 6.3±0.4 for CO2, and 3.9±0.4 for bags. The IVP rate for flasks (95.0%) was greater (P = 0.01) than for CO2 and bags (90.8% and 85.0%, respectively), but it did not differ between flasks and CO2 for three boars (P > 0.05). In experiment 2, co-incubation was done as described for glass flasks in experiment 1. The IVP rate and PSP were compared for cryopreserved oocytes: either vitrified in open pulled straws (OPS), or frozen in cryotubes. Mean PSP was 5.4±6.5 and IVP ratewas 89.6%. Both PSP and IVP ratewere greater (P < 0.0001) for oocytes frozen in cryotubes (7.0±0.3% and 95.8%, respectively) than those frozen in OPS (3.7±0.3% and 83.4%, respectively), with no differences found for three boars (P > 0.05). In summary, successful IVP of swine oocytes by homologous spermatozoa can be achieved using gametes incubated in glass flasks and oocytes frozen in cryotubes.pt_BR
dc.description.provenanceSubmitted by Ubirajara Cruz (ubirajara.cruz@gmail.com) on 2010-10-08T13:35:53Z No. of bitstreams: 1 AnimReprodSci - Macedo et al 2010 - In vitro penetration of swine oocytes - distinct systems for gamete co-culture and oocyte.pdf: 171471 bytes, checksum: fd64dbbbf2b8ac63133b0de9dab69018 (MD5)en
dc.description.provenanceMade available in DSpace on 2010-10-08T13:35:53Z (GMT). No. of bitstreams: 1 AnimReprodSci - Macedo et al 2010 - In vitro penetration of swine oocytes - distinct systems for gamete co-culture and oocyte.pdf: 171471 bytes, checksum: fd64dbbbf2b8ac63133b0de9dab69018 (MD5) Previous issue date: 2010-02en
dc.language.isoen_USpt_BR
dc.publisherElsevierpt_BR
dc.subjectIn vitro penetrationpt_BR
dc.subjectOocytespt_BR
dc.subjectCo-incubationpt_BR
dc.subjectVitrificationpt_BR
dc.subjectSwinept_BR
dc.titleIn vitro penetration of swine oocytes by homologous spermatozoa: Distinct systems for gamete’s co-incubation and oocyte’s cryopreservationpt_BR
dc.typeArticlept_BR
Aparece nas coleções:Departamento de Patologia Animal: Artigos de periódicos



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