Please use this identifier to cite or link to this item: http://guaiaca.ufpel.edu.br:8080/handle/123456789/114
metadata.dc.type: article
Title: Cryprotectant effect of trehalose and low-density lipoprotein in extenders for frozen ram sperm
metadata.dc.creator: Tonieto, Rafael Adolfo
Goulart, Karina Lemos
Gastal, Gustavo Desire Antunes
Schiavon, Raquel Schiavon
Deschamps, João Carlos
Lucia Júnior, Thomaz
Abstract: This study tested trehalose and low-density lipoprotein (LDL) as cryoprotectants in extenders for frozen ram semen. In the first experiment, the extenders were Tris, with 20% egg yolk (E1-1); E1-1 with 5% glycerol (E1-2); E1-1 with 100mM trehalose (E1-3); and E1-1 with 100mM trehalose and 5% glycerol (E1-4). Sperm motility and membrane integrity of the E1-2, E1-3 and E1-4 extenders were greater than for E1-1 (P < 0.05), but acrosome integrity following cryopreservation did not differ. In the second experiment, the extenders were Tris, with 20% egg yolk and 100mMtrehalose (E2-1); Tris with 8% LDL and 5% glycerol(E2-2); Tris with 8% LDL and 100mM trehalose (E2-3); and Tris with 8% LDL, 100mM trehalose and 5% glycerol (E2-4). Sperm membrane integrity was lowest for the E2-1 extender (P < 0.05), but similar for extenders including LDL. Sperm motility post-thawing was highest for E2-2 and E2-3 extenders (P < 0.05), but acrosome integrity did not differ. Thus, extenders including trehalose and LDL as cryoprotectants recorded a post-thawing ram sperm quality similar to that achieved when using conventional cryoprotectants.
Keywords: Extender
Frozen semen
Ram
Publisher: Elsevier
Citation: TONIETO R.A. et al. Cryprotectant effect of trehalose and low-density lipoprotein in extenders for frozen ram sperm. Small Ruminant Research, v. 93, p. 206-209, 2010
URI: http://hdl.handle.net/123456789/114
Issue Date: 9-Jun-2010
Appears in Collections:Departamento de Patologia Animal: Artigos de periódicos



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