Obtenção, caracterização e aplicação (in vitro) de nanoscaffold injetável funcionalizado com substâncias bioativas

Abstract

The development of scaffolds for tissue engineering should be biocompatible, where the degradation products should not be cytotoxic, tumorigenic, nephrotoxic or cause any toxic effects to the body. Despite the widespread use in tissue engineering, there is an effort to improve the functionality of this material for future applications. Important parameters should be observed in connection with properties that could influence the behavior of cells: pore size, pore shape, and surface treatment, for example. Among the materials used for the production of scaffolds, natural polymers have been the most widely used, given its high design flexibility and degradability. Scaffolds using nano-materials allow a greater similarity to natural extracellular matrix and these systems could be combined with biological inducers (eglectins) to address specific biological responses. The aim of our research is to obtain an alginate hydrogel functionalized nano/micro fiber based hydroxyapatite and titanium dioxide as well as bioactive substances (lectins), to the application in vitro for later use in the pulp tissue regeneration