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dc.creatorMacedo Júnior, Milton Carvalho
dc.creatorDeschamps, João Carlos
dc.creatorLucia Júnior, Thomaz
dc.creatorBordignon, Jonas
dc.creatorSerret, Carolina Gonçalves
dc.creatorRambo, Gissele
dc.creatorPivato, Ivo
dc.creatorSchmitt, Eduardo
dc.date.accessioned2010-10-07T12:40:02Z
dc.date.available2010-10-07T12:40:02Z
dc.date.issued2006
dc.identifier.citationMACEDO JÚNIOR, Milton C. et al. In vitro penetration of fresh and vitrified swine oocytes by homologous spematozoa using different incubation systems. Animal Reproduction Science, v. 92, p. 334-348, 2006pt_BR
dc.identifier.urihttps://guaiaca.ufpel.edu.br/handle/123456789/99
dc.description.abstractThe present study consisted of two experiments. In the first one, ejaculates from four boars were used to compare in vitro penetration (IVP) rates of fresh and vitrified swine oocytes by homologous spermatozoa in four treatments: fresh oocytes in conventional incubation (CO2 incubator) (FC), vitrified oocytes in conventional incubation (VC), fresh oocytes in submarine (bag) incubation (FS) and vitrified oocytes in submarine incubation (VS). The IVP rates for FC, VC, FS and VS were 46.5, 44.3, 36.9 and 33.1%, respectively. Analysis through Chi-square tests identified no differences in IVP rates between FC and VC and between FS and VS (P > 0.05), but IVP rate for FC was greater (P < 0.05) than those for both FS and VS. Besides IVP rate for VC did not differ (P > 0.05) from those for FC and FS, but it was greater than that for VS (P < 0.05). Logistic regression analysis identified differential effects of treatments dependant on individual boars. The second experiment evaluated the influence of semen storage period on the semen quality of the two boars associated with greater IVP rate in the first experiment. Semen quality was estimated by IVP rate using the VC treatment and by the following methods: sperm motility, sperm morphology, hypoosmotic swelling test (HOST) and thermal stress test (TST). According to analysis using Chi-square tests, IVP rate did not differ (P > 0.05), for the first boar, between 0 (100.0%) and 24 h of semen storage (98.1%) nor after 48 and 72 h (66.0 and 59.3%, respectively), but IVP rates were greater during the 0–24 h period compared with the 48–72 hpt_BR
dc.language.isoen_USpt_BR
dc.publisherElsevierpt_BR
dc.subjectSwine in vitro penetrationpt_BR
dc.subjectFresh and vitrified oocytespt_BR
dc.subjectSpermatozoapt_BR
dc.subjectIncubation systemspt_BR
dc.titleIn vitro penetration of fresh and vitrified swine oocytes by homologous spermatozoa using different incubation systemspt_BR
dc.typearticlept_BR


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