Efeito do exercício passivo em parâmetros bioquímicos e na atividade de ectonucleotidases em plaquetas de pacientes com Acidente Vascular Cerebral internados em Unidade de Terapia Intensiva

Abstract

Platelet activation and aggregation are critical in the pathogenesis of stroke. The aim of this study was evaluated the ectonucleotidase activities and biochemical parameters in platelets and serum from stroke patients hospitalized in the unit care intensive. The population studied consisted of the 18 cerebral stroke patients and 18 healthy individuals as control group. Passive mobilization was performed in stroke patients during seven days and the blood was collected in the first and seventh day. In each day the blood was collected before exercise session (time 0) immediately after exercise session (time 20) and 40 minutes after the application of passive intervention (time 60). The blood was used for platelet and serum preparation. In platelets was evaluated the NTPDase, 5´-nucleotidase and adenosine deaminase (ADA) activity an in serum biochemical parameters such as urea, uric acid, cholesterol, triglycerides, AST, ALT, creatinine and C-reactive protein. The results demonstrated an increase in the ATP hydrolysis and a decrease in the ADP and AMP hydrolysis in platelets from stroke patients when compared to control group (P≤0.05). No alterations were observed in ADA activity. After seven days the exercise passive was observed an increase in the NTPDase activity using ADP as substrate. No change was observed in the ATP and AMP hydrolysis. In relation the ADA was observed an increase in this enzyme activity in platelets after seven days the passive exercise only in the time 20. In relation the biochemical parameters our results showed a significant decreased only in the ALT and levels of C-reactive protein in serum of stroke patients after seven days of passive exercise. These results suggest that ectonucleotidase activities alterations in the platelet may contribute to vascular complications in the stroke and that passive exercise can be important in thrombosis mechanisms because it increase the ADP hydrolysis, the main agonist of platelet aggregation.