Desenvolvimento e caracterização de curativo nanoestruturado com liberação controlada de monopersulfato de potássio

Abstract

The use of biomaterials, mainly biopolymers, loaded with active compounds has the potential to interact with injured tissues, favoring tissue repair. The objective of this work is to synthesize and characterize a film based on nanochitosan (NQTS) and nanofibrillated cellulose (CNF) as a gradual delivery system of potassium monopersulfate (Oxone®, OXN) for use as a wound dressing. Thus, three dressing formulations were prepared: OXN 0% (Control group), OXN 0.5%, and OXN 1%, differing only in terms of the amount of OXN, the first without addition, the second with 0.5% (w/v) and the third with 1% (w/v). NQTS (0.4% w/v) and deionized water (DI) were heated to a temperature of 60°C and mixed for 30 min then CNF (0.3% w/v) was added to the solution under constant magnetic stirring and temperature for more 30 min. Heating was stopped and OXN was added to the solution and mixed for more 30 min. For the formation of films, the solution was poured over silicone plates and placed in oven at 50°C overnight. For its characterization, Infrared spectroscopy analysis with Fourier Transform Attenuated Reflection mode (FT-IR/ATR), scanning electron microscopy, thermogravimetric analysis, active compound release kinetics, and biological tests of cell viability and antimicrobial activity were performed. Nanostructured films having a good OXN delivery system were synthetized, the active compound was delivered slowly over the evaluated time (average delivery of 4% OXN every 30min of exposure). TG analysis proved that the films are thermally stable about its working conditions. The OXN 1% film completely inhibited the growth of the Escherichia coli bacteria proving to have excellent biological properties. The 0% OXN and 0.5% OXN samples show good biocompatibility. Therefore, it is possible to conclude that the films for application as a dermatological dressing obtained in the present study demonstrated viability about the characterizations, demonstrating an antimicrobial effect when added with potassium monopersulfate compound.