Nanoresveratrol diminuiu EROs e LPO e aumenta motilidade progressiva em espermatozoides bovinos descongelados

Abstract

Semen cryopreservation has been increasingly used for both scientific studies and practical application. This system presents some problems which seek a better understanding and resolution in a practical way, this can be with the use of compounds which can dribble and combat possible damaging mechanisms to the cells. Resveratrol is a polyphenolic compound with antioxidant, anti-inflammatory and anti-tumor activity, yet its consumption helps prevent heart disease, diabetes and obesity. In order to improve delivery and better absorption, resveratrol was nanoencapsulated and tested in cryopreserved sperm cells, both in its nano form (LNC-Res) and its free form (Res), at concentrations of 5, 25, 50, 100, 200 and 400μM. After thawing, spermatozoa were incubated for 15 and 60 minutes, after which their parameters were analyzed: membrane integrity, DNA fragmentation, acrosomal reaction, mitochondrial functionality, reactive oxygen species (ROS) and lipid peroxidation (LPO). The analyzes were performed in flow cytometry and kinetic analyzes in the CASA system, where the total and progressive motilities were observed. Therefore, the results showed that in the membrane integrity analyzes, after 60 min there was a reduction in the intact membrane levels at the concentrations of 25, 50, 100, 200 and 400 μM. In the analysis of DNA fragmentation no statistical difference was detected in relation to the control, which is in agreement with the results obtained in the analysis of OLP and ROS where both showed a decrease in 15 min and in the analysis of ROS a statistical difference between all treatments and control. The functionality of the mitochondria observed a statistical difference between LNC-Res in concentrations of 50 and 100μM when compared with 400μM of Res after 60 minutes. With these results, it can be seen that resveratrol did not induce toxicity to bovine sperm cells and it was observed that this compound in its nanoencapsulated form stimulated the intracellular antioxidant machinery generating a protection for them. In this sense, LNC-Res may become a promising pharmaceutical form for the drug in question.