Brucelose bovina: avaliação de testes sorológicos após aplicação de levamisole

Abstract

The aim of this study was to describe false-positive serological reactions (FPSR) in a study of 989 serologically negative cattle from farms with and without a history of abortion Brucella spp, as well as evaluating the links between some individual or herd factors and the occurrence these FPSR. We use the test Buffered Acidified Antigen (AAT) in all animals and reagents in the AAT first blood sample, if confirmed by tests 2 Mercaptoethanol (2ME) and Fluorescence Polarization Assay (FPA) were referred to stamping. Non reagents were inoculated on day zero with Levamisole (782) and two control groups (207) with saline (100) and Ivermectin (107). The two control groups were negative in all tests. The levamisole group had an overall incidence (IT) of 1.41 % of FPSR. The Relative Risk (RR) of FPSR spring was 23.42 times more likely than in other seasons. Fraction Etiologically Risk (FER) showed that 95.73 % of FPSR occurred by exposure to levamisole plus the spring effect. The incidence of animals with FPSR with Body Condition (CCr) poor was 10.34 % and with good CC (CCb) was 6.1 %. The RR of FPSR with CCr was 9.74 times more likely than in CCb. The FER showed that 89.73 % of FPSR occurred by exposure to levamisole more CCr. The use of levamisole as an immunostimulant and anthelmintic remains recommended, however, a grace period of at least 15 days must be observed between application of the product and make the diagnosis for bovine brucellosis.