Caracterização citotóxica e cicatricial dos extratos de Triticum aestivum e Copaifera sp.

Abstract

Wounds in the skin area occur frequently in small animals. Consequently, the therapeutic validation of healing products in preclinical studies are critical to conducting clinical trials in the target species. This study aimed to perform preclinical studies to determine the healing action of different doses of T. aestivum extracts and Copaifera sp. The tests evaluated the cytotoxicity of phytotherapic and the in vivo healing of skin wounds for a period of 21 days. In the in vitro experiment with T. aestivum it was observed that a dose of 2mg/mL was cytotoxic to the cells and that higher doses showed cell viability. The wounds made in rabbits treated with the aforementioned dose presented a superior result to those treated with a 10 mg/mL dose. This outcome was feasible due to the fact that during the treatment with the lower dose there was not an excessive formation of collagen, thus allowing a standard epithelial tissue formation. It was found in the in vitro study with Copaifera sp. that the safe doses are the equal and less than 0.01%. In the in vivo assay with rats that wounds treated with copaiba at 0.1% and 0.01% have their healing process accelerated in the early days due to the higher wound contraction. However, in the scar quality, these wounds showed less results to those treated to saline and vaseline. It is possible to conclude that doses of T. aestivum extracts to 2 mg/mL. In relation to the copaiba oil resin, an efficient dose for clinical trials was not achieved.