Utilização de biorreatores de imersão temporária na micropropagação de batata-doce

Abstract

The use of plant micropropagation technique has become a common tool between producers of certified plants. But the conventional micropropagation system is still broadly used without giving due importance to the temporary immersion system (TIS). The objective of this study is to present a cheaper alternative production costs and simplification of in vitro plants of work, through the assembly and use of temporary immersion bioreactors and use of a new double solenoid valve model five open center routes negative. In this study, we present a model of TIS, built by hand, that when compared to similar manufactured equipment showed results with equivalent productivity, proving to be a very useful equipment in automation for production of in vitro plants. At the same time, we developed a microbiorreactor using glass containers of small capacity, in order to work with low availability of vegetal material explants , which can be used for scientific research successfully, as confirmed in preliminary studies with cultures of blackberry, raspberry and apple. The main plant specie used in this experiments with the bioreactor, sweet potato, has great popular appeal due to its contribution as a supplier of food carbohydrates, amino acids and vitamins and had not yet been tested in this micropropagation system. The results obtained with multiplication in bioreactors were similar to rates in conventional system, showing that the use of TIS protocols must be optimized, especially with regard to the time and number of immersion, composition of culture media and photon flux density. With the conventional system, there wasn't observed influence differential between white-cold fluorescent lamps and LED and 7.5 g L-1 sucrose showed a similar result to 15 and 30 g L-1 in the variables studied, indicating that this species is not very exigent about external addition of sugars in the culture medium, and perhaps it could be a plant material suitable for studies of photoautotrophic micropropagation. Finally, the equipment now available in Plant Culture Laboratory may be used in the development of new scientific research related to physiology, anatomy and production of secondary metabolites.