Desenvolvimento de curativos flexíveis de alginato/celulose/hidroxiapatita sensíveis à presença de óxido de grafeno e chalconas

Abstract

The alginate hydrogel is used for different purposes, among them, tissue engineering and as curatives for healing. Due to its low properties, there is an ongoing effort to overcome its limitations, including other materials is an alternative to improve its properties. Therefore, the objective of this study was to perform an alginate hydrogel membrane with cellulose, hydroxyapatite, graphene oxide and chalcones with antimicrobial properties. To obtain the pulp, alternative sources of agroindustrial residues were used, from the stem of the banana peel. Cellulose was coated with hydroxyapatite. For the coating, microwave assisted hydroxyapatite was synthesized using calcium nitrate tetrahydrate, phosphoric acid, 1,2-ethylenediamine and cellulose solution with variations of (0.25, 0.5 and 1 g cellulose), 140º 5 minutes. To prepare the alginate hydrogel membranes was used sodium alginate and calcium sulfate P.A Dihydrate and deionized water. In the modified alginate was added hydroxyapatite or cellulose precipitated with hydroxyapatite, hydroxyapatite with graphene oxide, hydroxyapatite cellulose and graphene oxide and chalcones. After complete homogenization, the samples were frozen and lyophilized, the chalcone concentration was 0.0015 g dissolved in 0.2 ml DMSO for each 10ml final solution. Samples were frozen and lyophilized. The samples were characterized by: BET (Brunauer Emmett Teller), XRD (Ray Diffraction), FITR (infrared spectrometry), SEM (Scanning Electron Microscopy), FEG (Electron Emission Microscopy), TG (Thermogravimetric Test) and cell viability test for cellulose coated with hydroxyapatite, and microbiological test with Candida albicans and parapsilosis for membranes with GO and with the positive bacteria Staphylococcus aureus (S. aureus) for chalcone variations.