Propriedades físico-químicas e antimicrobiana de cimentos endodônticos experimentais contendo metacrilatos metálicos

Abstract

The properties of endodontic sealers are important for the quality of filling of the root canals and, later, in the endodontic prognosis. However, no material has all theproperties of an ideal endodontic sealer, so new materials are being created to improve its physicochemical and biological characteristics. The objective of this study was to evaluate the physical, chemical and antimicrobial properties, and shelf-life of a dual-reactive experimental sealer after the incorporation of dibutyltin methacrylate (Sn4+) (ETs) or calcium methacrylate (Ca2+) (ETs), in a concentration of 2%. AH Plus was used as a commercial reference. The pH and ion release were measured using a pH meter and a microwave induced plasma optical emission spectrometer. The dimensional stability was evaluated after 30 days in accordance with ISO 6876. Inhibition of biofilm growth was evaluated by laser scanning confocal microscopy (CLSM). The biofilm viability analysis was performed using the SYTO 9 technique. The shelf-life was evaluated through tests of degree of conversion and film thickness at the times: immediate, 1 month and 2 months. For statistical analysis, ANOVA and Tukey post hoc test were used, and a significance level of 5%. All sealers tested showed a gradual reduction of pH over 30 days. The ECs sealer showed the highest release of calcium and ETs presented the highest release of tin in 30 days. The ETs sealer showed better dimensional stability over time compared to other endodontic sealers. ETs revealed better antibiofilm potential after 15 days compared to controls. The degree of conversion was reduced after the shelf-life. Regarding film thickness, all materials were in accordance with ISO 6876 specifications, except ECs immediate (100 μm) and ETs in the first month of aging (170 μm). It was concluded that the physicochemical properties of the experimental endodontic sealers containing calcium and dibutyltin methacrylate were not drastically altered and the antibiofilm effect of the cements was improved.