Efeito do butafosfan e cianocobalamina sobre a produção de oócitos e embriões in vitro de vacas Jersey

Abstract

The aim of this study was to evaluate the effects of supplementation of butafosfan and cyanocobalamin (BTC) in Jersey cows on the in vivo oocytes recovery and in vitro embryos production. Thirty-six cows were homogeneously divided into two identical experiments, performed at different periods. In each experiment, the BTC group (n=9) that received 4500 mg of butafosfan and 2.25 mg of cyanocobalamin and the control group (n=9) that received placebo (NaCl 0.9%). The treatment was performed on days 0, 5, 9, 14, 19, 23, 28, 33, 37 and 42, concurrently with blood samples collection for analysis of phosphorus, cholesterol, urea and non-esterified fatty acids (NEFA). During the same period, each cow was subjected to 4 sessions (days 0, 14, 28 and 42) of ovum pick up (OPU) and in vitro embryo production. In exp. 1, the BTC group had a tendency (P=0.08) to lower cholesterol blood concentration and a higher number of embryos produced at IVF 28 (P=0.02). In exp. 2, BTC group tended (P=0.09) to reduce concentrations of NEFA and to increase (P=0.07) the production of grade II oocytes. Moreover, at the IVF42 was observed reduction (P=0.01) of the blood NEFA concentrations and increases (P≤0.05) of the number of punctured follicles, oocytes retrieved and mature oocytes in the BTC group. These results indicate that supplementation with butafosfan and cyanocobalamin in dairy cows improves the energy status and increases in vitro production of oocytes and embryos.