Identificação de marcadores bioquímicos e proteínas de fase aguda no líquido amniótico de éguas no momento do parto

Abstract

The analysis of amniotic fluid is useful for understanding the biochemical process involving the fetal-placental unit. The aim of this study was to evaluate biochemical profile and acute phase proteins in the amniotic fluid of healthy mares during labor. The collection of amniotic fluid was performed by amniocentesis method, once exposed to the amniotic sac in the first stage of labor, by direct puncture. It has been observed forty Thoroughbred pregnant mares in Southern Brazil. The biochemical composition was evaluated using commercially kits and proteinogram was measured by eletrophoresis in poliacrylamide gel (SDS – PAGE). The results of mean and standard error were: pH 7.70+0.02; glucose 1.22+0.08; alkaline phosphatase 30.45+ 2.67 (IU/L); gamma – GT 4.6+0.62 (IU/L), urea 33.59+2.34 (mg/dL), creatinine 5.10+0.59(mg/dL), sodium 54.35+2.51 (mEq/L); potassium 1.71+0.12 (mEq/L), chloride 72.09+5.94 (mEq/L); calcium 3.86+0.23 (mg/dL); osmolarity 277.32+17.76 (mOsml/L) and total protein 26.04+4.39 (mg/dL). The osmolarity was correlated with ions sodium (R=0.60), potassium (R=0.55) and calcium (R=0.53). Twenty-two acute phase protein were detected and that inflammatory proteins albumin and transferrin have been identified in more than 97% of the evaluated mares. It was possible to identify biochemical markers and inflammatory proteins in the amniotic fluid of healthy full term mares. Only the transferrin presented among different age groups, with lower concentrations in the older group of mares.