Atividade Antioxidante de Chalconas Derivadas do 2-Acetil-tiofeno.

Abstract

In this study we describe the synthesis of six chalcones synthesized from 2-acetyl-thiophene and evaluate its antioxidant activity in vitro through the test of radical 2,2-diphenyl-1-picril-hidrazila (DPPH•). After, the compounds were subjected to the following tests: thiobarbituric acid reactive species (TBARS, lipid peroxidation), formation of carbonyl groups (oxidative damage to protein) and total thiol content in cerebral cortex and in liver of rats. Among the compounds evaluated, chalcone B showed antioxidant activity on DPPH• test (EC50 of 40.4 μM). However, all chalcones tested significantly reduced lipid peroxidation in cerebral cortex of rats. In contrast, the content of carbonyl and total thiol groups has not been modified in the cerebral cortex and liver. The compound B reduced the extent of TBARS on all concentrations tested (1 to 300 μM) in a dose-dependent manner, being considered the best antioxidant evaluated in this study.