Biomodelos e simuladores: da pesquisa ao ensino

Abstract

The doctoral period is a time when the future doctor must experience the three pillars of education: research, extension and teaching. Phytotherapy research stands out in the scientific scenario, but many products do not have their efficacy and safety validated. In this sense, the effects on sperm parameters of the topical use of copaiba oil (OC) ointment, which is used for the treatment of inflammatory skin lesions, were studied. Animals treated with 0.01% and 0.1% OC for 7 days increased the number of damaged membrane cells (79.6% and 68.9%) when compared to the control group (43%). After 14 days (D14) of treatment, an increase in the percentage of damaged DNA cells was observed in the 0.01% (27.8%) and 0.1% (23.7%) treated groups with copaiba. control group (0%). Showing that topical use of OC can decrease sperm quality over the period and concentrations studied. However, early identification of the toxicity of new substances through the development of alternative animal methods is a constant pursuit, guided by the 3R's Principles. A screening assay has been proposed to identify the toxicity of different doses of swine sperm cell ATP compared to cells of the ZFL strain by assessing cell kinetics, structure and metabolism. Swine sperm were shown to have a high sensitivity to the toxic effects of the tested agent, so it was possible to detect changes in plasma membrane and reactive oxygen species production, data not found in cell line evaluations. In the field of education, the development of the teaching project "Production of alternative didactic resource for the Veterinary Medicine course" was elaborated from a canine dummy capable of making possible the abdominal and vaginal palpation of a pregnant female. This material enriched the practical classes of the Obstetrics and Mammary Gland discipline of the Veterinary Medicine course, providing greater security for students to perform the maneuver in a future patient. Being a viable alternative to the use of animals. Regarding the university extension activity, the First Cycle of Prenatal Lectures on Small Animals, had as its main objective to disseminate and propagate information about the most differentiated themes about obstetrics in small animals and how to overcome the obstacles that still persist about this process. The lectures were given by expert veterinarians exposing divergent views on the complexity of the topic addressed.