Exigência de lisina e estimativa dos principais aminoácidos essenciais com base no conceito de proteína ideal para Odontesthes bonariensis

Abstract

The pejerrey Odontesthes bonariensis is a kind of great importance in artisanal fishing in southern Rio Grande do Sul, but on the face of fishing in this region is diminishing fisheries resource. This species arouses great interest for cultivation, but the development of low Atherinicultura in Brazil, due mainly to a lack of studies on the techniques of cultivation of this species, and the lack of studies on the nutritional requirements. The aim of this study was to determine through a purified diet nutritional requirements of essential amino acids for pejerrey Odontesthes bonariensis. The experiment was conducted in the Laboratory of Ichthyology Department of Animal Science, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, occurred in a 60-day period consisted of 24 boxes each containing polyethylene 7 animals randomly distributed. The experimental design was completely randomized experiment had 6 treatments and 4 replications. The statistical analysis of mean, standard deviation, and mean comparison regressions were performed using the statistical software, SAS 9.0 and Bioestat. The six purified diets) the basis of albumin and gelatin were isoproteic (40% CP) and isocaloric 3500 of digestible energy, only varying concentrations of lysine (1.1, 1.4, 1.7, 2.0; 2.3 and 2.6%). Based on the regression equation resulting from the highest level of lysine to the species of kingfish O. bonariensis which was 1.95% of the diet synthetic lysine and lysine 4.87% of total protein. We performed the analysis of amino acids body of the fish, so through the computation requirement in the essential amino acid lysine requirement = x (rate A / E / 100). It was determined the requirement of other amino acids, and, histidine 0.60%, arginine 1.21%, 0.74 of valine, methionine 0.93%, isoleucine 0.65%, leucine 1.30% , phenylalanine 0.72%, lysine 1.79 and 0.73 threonine.