Caracterização físico-mecânica e potencial biológico de materiais odontológicos contendo extrato pirolenhoso obtido de Eucalyptus grandis

Abstract

The present study consists of the development of new dental materials (varnish and adhesive system) containing Eucayiptus grandis pyroligneous extract of highly purified. Initially, a systematic review of the scientific literature on the antimicrobial potential of pyroligneous extract was carried out, as well as a technological prospection through patent search. This project consisted of pre-clinical trials of two experimental dental materials and was divided into two stages. The objective of the first step was to investigate the antimicrobial and cytotoxic potential of six samples of a chitosan film containing E. grandis pyroligneous extract, aiming its application for dental use. The second step involved the formulation of an adhesive system containing the E. grandis pyroligneous extract at concentrations of 1, 5 and 10%. The direct contact antimicrobial tests, cytotoxicity test and physical-mechanical test of these formulations were performed. In the bibliographic and technological monitoring, the most predominant studies were in vitro, and demonstrate the antimicrobial action for oral pathogenic microorganisms. The experimental materials tested showed antimicrobial activity (Candida albicans, Streptococcus mutans and Lactobacillus acidophilus) at low concentrations (chitosan film – 7.500μg at 1.500μg and 1%, 5% and 10% adhesive system), incorporation of the E. grandis pyroligneous extract into the system adhesive did not negatively influence the mechanical properties of the material and was not shown to be cytotoxic against commercial fibroblast cells from NIH / 3T3 lineage mice. Although clinical studies are required to confirm the effectiveness of these materials in the prevention and control of oral pathologies, the systematic review and the laboratory study have demonstrated that there is evidence of antibacterial activity of these materials in vitro.