Uso de lectinas nativas e recombinantes de patade-vaca (Bauhinia forficata e Bauhinia variegata) e banana (Musa acuminata) no controle da formação de biofilme em cateter por isolados de Klebsiella pneumoniae produtores de carbapenemases (KPC)

Abstract

Biofilms are complex microbial communities that reside in a matrix of exopolysaccharides and adhere to biotic and abiotic surfaces. In the hospital environment, they are typically the cause of chronic infections and healthcare associated infections (HAI) associated with medical devices. Biofilm allows microorganisms to withstand adverse conditions and environmental stress, including the host's immune system and the action of antimicrobials. This characteristic makes therapy difficult and justifies the search for molecules with action to control biofilm formation. In this context, we highlight lectins, proteins capable of binding specifically to carbohydrates present on the surface of the bacteria and triggering their action. Thus, the objective of this work was to verify the potential of control action on biofilm formation by native and recombinant lectins, isolated from the plants Bauhinia forficata, Bauhinia variegata and Musa acuminata, against Klebsiella pneumoniae isolates that produce carbapenemases. Several tests were carried out to evaluate biofilm formation by the isolates, such as the Congo Red test, in vitro biofilm formation, and standardization of the method for evaluating biofilm formation in catheters, as well as tests to assess the ability of lectins in control of biofilm formation, as minimum inhibitory concentration and minimum bactericidal concentration. The lectins demonstrated bacteriostatic activity and the ability to reduce biofilm formation in hospital-use catheters in the evaluated isolates. The data generated in this study suggest that lectins have potential in the development of an antibiofilm formulation, but further studies will be needed to elucidate the mechanisms of action, combination therapy with antimicrobials and therapeutic strategy.