Detecção de DNA de Trypanosoma spp. e Leishmania spp. em órgãos de javali (Sus scrofa)

Abstract

The protozoan of Trypanosomatidae Family have great importance in one health because they are considered reemerging zoonosis, and the wild boar (Sus scrofa) has shown to be a potential disseminator of these agents. The goal of this dissertation was to evaluate the presence of Trypanosoma spp. and Leishmania spp. in wild boar organ tissues. For the development of the study, 75 samples of 25 animals were analyzed, from authorized hunting, and the aliquots were sent from the (Laboratory of Veterinary Microbiology and Immunology of the Farroupilha Federal Institute of Education (LAMIVET/IFFar) to the Laboratory of Parasitic Diseases of the Federal University of Santa Maria (LADOPAR/UFSM) for molecular analysis. After DNA extraction, the material was submitted to the PCR technique and genetic material for Leishmania infantum (4%) and Leishmania amazonensis (4%) was identified in the spleen and liver, respectively, and for Trypanosoma spp. (44%) in eleven heart samples. The data present in this study reveal the importance of population control in wild boar and how these animals can serve as a reservoir for diseases of public health relevance.