Expressão de genes WRKY e alterações morfológicas em arroz sob estresse por submergência

Abstract

The growth of the world’s population has contributed for a greater demand of rice while environmental alterations caused by men contribute to an increase of rainfall. This conditions create a hypoxia environment and restrict the production of rice. Under these conditions, plants with morphological adaptations and alterations in molecular level. Thus, this work had as its objective to evaluate the morphological and anatomical effects in rice cultivars Oryza sativa L. submitted to hypoxia and to verify the expression profile of genes of complex and simple regulation of WRKY transcription factors involved in the response of stress by hypoxia. The results obtained demonstrated that morphological and anatomical alterations occur in cultivars of irrigated rice when submitted to stress by hypoxia, manly in their root system. Furthermore, the aerenchyma is more developed in the cultivars Epagri 108 and Nipponbare when submitted to stress by hypoxia. In the study of genic expression through the qRT-PCR technique, it was possible to observe that the WRKY genes responded to stress by hypoxia but it not possible, for this stress, to verify a difference between the response of the expression profile of genes of simple and complex regulation in seedlings of rice under stress by hypoxia.