Expressão diferencial do gene HPS no tegumento de sementes de soja

Abstract

The objective of this study was to evaluate the gene expression of HPS in the seed coat of four soybean genotypes at different sampling times after anthesis. The experiment was conducted in greenhouse at Estação Terras Baixas of Embrapa Clima Temperado and Laboratório de Sementes e Biotecnologia (UFPel / FAEM) in Capão do Leão city - RS, in a randomized complete block design with three replicates, and treatments arranged in a factorial model. Were used four contrasting genotypes, two cultivars of yellow coat (COODETEC 202 and BMX Potência RR) and two genotypes of black coat (Tegumento Preto and IAC). Seed coat samples were collected along the developing soybean seed, intervals for 5 days, between the periods 25-55 days after anthesis. Proceeded the RNA extraction from the seed coats, followed by obtaining the cDNA for further analysis of qRT-PCR, to quantify the relative accumulation of gene transcripts HPS. IAC 222 showed higher values of relative quantification in all periods, with the highest value found in the collection of 35 days after anthesis. The relative accumulation of transcripts of the HPS gene in the seed coat of soybean differs between the periods evaluated, as well as between genotypes.