Teste de tetrazólio para avaliação de sementes em feijão caupi: concentração do sal e período de coloração.

Abstract

The increase in area sown in off-season with cowpea (Vigna unguiculata (L.) Walp.), In the Brazilian Midwest, caused an increase in good quality seed demand and high force. The tetrazolium test is one of the options available to assess the quality and vigor and, although there is standardized methodology by the Rules for Seed Analysis (Brazil, 2009), the methodology proves to be inefficient and it is necessary to adapt it to obtain results reliable. Thus, work was conducted in order to study different tetrazolium salt concentrations and staining time in evaluating the physiological potential of cowpea seeds. They used seeds of BRS Guariba (Class White Smooth), BRS Novaera (White Class Rough) and BRS Pajeú (Class Mulato Straight), each represented by a lot, produced in 2014. The second-crop seeds were submitted to the test germination and seedling vigor classification. The tetrazolium test has been conducted using three different concentrations of tetrazolium salt (0.500%, 0.075% and 0.050%) with exposure time of 90 minutes, 150 minutes and 960 minutes. The data were submitted to variance analysis and when presented significant difference, the means were compared by Tukey test at 5% and subjected to Pearson correlation analysis, correlating the test in sand and different times and concentrations tested in the test tetrazolium . The results showed that the reading of the tetrazolium test is possible in the three soaking times for the three solution concentrations, except in the concentration of 0.500% tetrazolium salt in time of 960 minutes. Keywords: