Propagação de Goji Berry (Lycium barbarum L.)

Abstract

The methods to acquire Goji Berry seedling has not been established in Brazil up to this date. Thereby, this study’s aim is to create a methodology to analyze seeds from this fruitful tree as well as to do its micropropagation. In the first experiment, it was intended to verify the germination temperature and to establish the first and last seed count. Therefore, four temperatures were tested: 20ºC, 25ºC, 30ºC and 20-30ºC and the emergency time at 25ºC. It was verified that the first count should be performed on the 3rd day after the germination test was installed and the last one should be performed on the 10th day. It was also seen that the temperatures of 25ºC and the alternated 20-30ºC are considered ideal for the germination test and that the alternated temperature has provided the highest value for the analyzed variables. The second experiment was performed at UFPel’s Laboratório de Propagação de Plantas Frutíferas, and it was used MS medium culture as medium base. In the in vitro multiplication phase, was verified the explants obtainment under photoautotrophic conditions using different types of sealing (cotton, aluminum and Parafilm®) and different concentrations of sucrose (0, 15, 30, 45 g L-1). Data was submitted to variance analysis using the F test (p≤0.05). Being verified statistical significance, the effect of the variation of sealings were compared by the Waller-Duncan test (p0,05) and concentrations by regression models (p≤0.05.It was concluded that the cotton-type sealing associated with the use of sucrose at the concentration of 30 g L-1 provided the highest results for the number of budding and length of the longest budding. This combination was also efficient in obtaining a higher number of leaves, but the highest value was obtained using Parafilm as sealing and the sucrose concentration of 45 g L-1. Vitrification was observed to a lesser extent in flasks sealed with aluminum. In the absence of sucrose in the culture medium, cotton has provided the highest averages of fresh and dry matter.