Metodologias alternativas para teste tetrazólio em sementes de soja

Abstract

The production and use ofhigh-quality seeds are basic factors for the success of soybean (Glycine max (L.) Merrill) crop. For such requirements to be achieved, the quality control system in the seed industry must be agile, versatile, and reliable, providing accurate and rapid results. Tetrazolium test allows obtaining information on seed viability and vigor, allowing the diagnosis of mechanical damage, deterioration due to moisture, and stink bug damage. The aim of this study was to define alternatives methodologies in the tetrazolium test in soybean seeds of TMG 1176RR, and TMG 133RR cultivars. The evaluations were tetrazolium test a) Pre-soak for 16 hours Pre-soak for 2 hours b) Pre-soak for 8 hours followed by pre-conditioning for 16 hours and TZ c) Pre-soak for 16 hours followed by pre-conditioning for 16 hours and TZ d) Pre-soaking for 24 hours followed by pre-conditioning for 16 hours and TZ e) Pre-soak for 72 hours followed by direct staining. Tetrazolium test is an efficient tool for quality control of soybean seeds. The Brazilian Rules for Testing Seeds methodology presents efficiency in the evaluation of the physiological quality of soybean seeds.