Hidrogel de alginato funcionalizado para utilização como sistema de entrega em Odontologia

Abstract

Innovative therapies have been evaluated for repair or regeneration of tissues and organs affected by disease or trauma. The drug release directly on the affected tissue can improve response to treatment, increasing the chances of obtaining therapeutic success. An alternative would be the use of alginate hydrogel, a copolymer derived from a natural biocompatible polysaccharide, because of their characteristics as biocompatibility, hydrophilicity and low cost, it has been used in a variety of applications in health. Used extensively as carrier of drugs and growth factor, its use can enable more biological treatments with the induction of events involving the repair or regeneration of tissues of interest. The aim of this work was divided into two parts: the first was to synthesize and characterize alginate hydrogel microspheres containing methacrylate radicals for use as delivery system. The alginate methacrylated was synthesized by 24 hours and product conversion was followed by spectroscopy of Fourier transform infrared (FTIR). The product obtained after purification was characterized by H1 NMR. Alginate microspheres were obtained by the spray-coagulation method in CaCl2 solution for cross-linking. Different formulations that ranged as far as methacrylate funcionalization, photoinitiators, and presence of chitosan, were tested. Cell viability assay was investigated and the mean diameter of the microspheres was obtained by optical microscopy. In the second stage of work was held the technological prospecting with the description of the invention patent for the development of biomaterials containing functionalyzed alginate microspheres with bioactive molecules. FTIR and NMR analyses confirmed the successful synthesis of alginate hydrogel containing methacrylate. Cell viability assay showed that all groups have low cytotoxicity, with similar results to the control and microspheres showed an average diameter of 20 µm. This study showed a simple and effective way to obtain alginate hydrogel microspheres containing methacrylate radicals, with a high potential for application as delivery system in dental materials.