Sinergismo dos probióticos Saccharomyces boulardii E Bacillus cereus var. Toyoi sobre a imunomodulação em camundongos.

Abstract

The combination between probiotics can generate a response higher or similar to the sum of the response obtained by each of them alone, hence, providing a synergistic effect. The yeast Saccharomyces Boulardii and Bacillus cereus var. Toyoi bacterium have got the beneficial property to modulate the host immune response. The aim of this study was to evaluate the influence of the combination between the probiotics S. Boulardii and B. Toyoi on the humoral and cellular response in mice, making use of the recombinant protein internalin A (rlnlA) of L. Monocytogenes as antigen model. Firstly, it was evaluated the resistance of these microorganisms co-administered to simulated adversities of gastrointestinal tract (GIT) of humans and to the passage through GI of mice. Later, it was evaluated the capacity of the combination of these microorganisms in stimulating the humoral and cellular response in mice. For this purpose, mice fed on diet containing both probiotics and alone were immunized with rlnlA, blood samples were collected in the days 0 and 29 and sera submitted to ELISA indirect for the titration of specific total lg, lgG1 and lgG2a. To evaluate cellular response, the concentration of interferon y (IFN-y) produced by splenocytes from the cultured and rlnlA stimulated mice has been assessed. Both probiotics evaluated alone and in combination, showed high survival rates to the GI simulation of humans and to mice GIT. The animals from the group in which S. Boulardii and B. Toyoi were co-administered showed lg total antibody titre and seroconversions significantly higher than the animals supplemented with each one of the probiotics alone. Besides, the immunization with rlnlA stimulated higher levels of lgG1 related to lgG2, resulting mainly in TH2 response, humoral reponse. In conclusion, the probiotics S. Boulardii e B. Toyoi acted synergistically, enhancing the specific humoral immune response against the rlnlA antigen, and they can be used in combination because there was no antagonism between themselves towards the survival to GIT of mammals.