Fitoquímicos e atividade metabólica de extratos de amora-preta (Rubus sp.) e de mirtilo (Vaccinium sp.) em ratos wistar.

Abstract

The body's normal metabolic activity contributes to oxidative stress with damage to DNA, RNA, proteins and other oxidizable substances. Hypercholesterolemic diet, stand out as an accelerating factor in the production of free radicals in living beings, contributing to the onset of degenerative diseases. Some bioactive compounds in blackberry (Rubus spp.) And blueberry (Vaccinium sp.) Have the ability to act as natural antioxidant food becoming able to minimize effects on the body caused by reactive oxygen species. Based on this context it was proposed to assess pulp concentrated, diluted (50% and 75%) and lyophilized blackberry and blueberry, through its physical and chemical characteristics, quantification of phytochemical compounds and their antioxidant potential. To evaluate the antioxidant in the body of Wistar rats, adult male, was used for mixed drinks blackberry and blueberry, with concentrations of 50% and 75% offered daily to biological models, which are fed diets normolipídicas ( AIN-93M) and hypercholesterolemic (plus 0.1% cholic acid, 1% crystalline cholesterol (w / w)). A total of 60 rats, Wistar strain, average weight of 275.29 ± 26.32 g, from the Vivarium of the Federal University of Pelotas, RS, these randomly divided into ten experimental groups, six per group, and five groups fed rations hyperlipidemic and five normolipídica diet, having a control group in each series. Eight groups received daily 5mL pulp blackberry and blueberry, at concentrations of 50% and 75%. We evaluated weight gain, feed intake, fecal and liver lipids, liver and serum lipid profile and liver weight. Dosed if the total cholesterol, triglycerides and liver and plasma. Was quantified in liver and frontal cortex, lipid oxidative damage (TBARS) and protein (SH), antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) test and non-enzymatic antioxidant defense (TRAP). The pulp had blueberry lowering effect on serum triglycerides, total cholesterol, LDL-cholesterol and VLDL-cholesterol, showing a significant influence on HDL in rats with normal and hypercholesterolemic. Results obtained in this study, we conclude that the fruit pulp from blackberry (Rubus spp.) And blueberry (Vaccinium sp.) Showed antioxidant activity, however, the intensity of this action was different for them. Standing out with the highest antioxidant potential in vitro, the blueberry.