Efeitos da supressão do gene tropinona redutase II sobre propriedades do amido e acúmulo de metabólitos secundários em batata silvestre (Solanum commersonii subsp.malmeanum Bitt.).

Abstract

The potato is use frequently as a model plant for carbohydrate metabolites. The wild species when compared to the cultivated (Solanum tuberosum), they excel for biotic and abiotic stress tolerance, lower reduce sugar content, and bigger total soluble solids. Therefore, the wild potatoes are use as study model and in cultivated potatoes breeding. However, it is known the wild species by the rule shows a high concentration of toxics compounds, such as glycoalkaloids, polyamines and alkaloids, being inappropriate as food. Therefore, strategies to suppress the genes that control the syntheses of this toxics components are of great interest, including for the its potential to promote syntheses alterations in others components naturally presents; as corn flour for example. In this study, the corn flour properties alterations and the secondary metabolites accumulation are describe for wild breeding potato (Solanum commersonii ssp. malmeanum) for the tropinone reductase II gene suppression. We observed alterations in the swelling power and solubility, in thermic properties, of pulp and the corn flour crystallinity extracted from the tuber, but without beads morphologic alterations. Furthermore, we observed increases in glycoalkaloids concentrations (α-solanine and α–chaconine) and polyamines (putrescine, spermidine and spermine). The calystegine rich wild potatoes extracts showed a excellent antimicrobial activity, being capable to inhibiting for 10 hours the mannitol and maltose sugar fermentation from Salmonella enteritidis and for 12 hours the galactose and maltose sugar fermentations from Listeria monocytogenes. This highlights the specific inhibition of microbial glucosidases, supposedly by the calistegianas action presents in the extracts.