Fragmentos de genes diferencialmente expressos durante a germinação/emergência do arroz sob estresse da profundidade

Abstract

Grown on every continent, rice (Oryza sativa L.) has great social and economic as well as being a model plant for studies of genetics and physiology of plants. Brazil is among the top ten world producers of this culture, and the Rio Grande do Sul is responsible for 60% of Brazilian production. Due to its versatility and adaptability to different soils and climates, it is the breeding of the search mechanisms on the resistance of plants to different biotic and abiotic stresses, high productivity and quality, and for this, absolutely the presence of genetic variability . In this sense, red rice accessions have been widely used because they have a range of as yet unidentified genes, and identification of the resource responsible for competition and morphological possible be brought into cultivated rice, makes this species attractive for the identification and characterization of genes and involved in the process of germination / emergence, in studies of differential expression. This study aims to identify differentially expressed fragments in the germination / emergence of red rice ecotype and two genotypes grown under stress depth (BRS Taim 7 representing the cultivated rice, the red rice ecotype FFS-45 and cultivar Nipponbare) . The lines were placed to germinate at 15cm depth. Samples were collected at 4 days, 7 days, 14 days and 21 days after sowing and soon after each collection was extracted RNA for construction of cDNA. To obtain the fragments of differentially expressed genes among genotypes, we used the technique of cDNA-AFLP, which were tested 16 primer combinations, which allowed the identification of 95 differentially expressed fragments, and 3 of these compounds showed no amplification. The analysis of the bands was performed by visualization on acrylamide gels, using the criterion of presence or absence of bands between the genotypes and the difference in intensity between fragments. By identifying these genes by sequencing study, characterization and cloning, will contribute to studies related to the identification of genes involved in germination and emergence of rice.