Análise do crescimento e identificação de loci potencialmente amplificáveis em peixe-rei.

Abstract

Aiming to provide technical and scientific allowance for defining the best animal model for pejerrey, to be used in a breeding program, two separate in a single project experiments were performed. In first experiment were examined the growth performance of Odontesthes bonariensis, Odontesthes humensis and hybrid (O. bonariensis X O. humensis) in intensive culture system for a period of 180 days, using the body weight (W), total length (Lt), condition factor (CF), allometric coefficient (b), specific growth rate (SGR) and estimates of body weight and maximum total length (Wmax, Ltmax) as indicators. All genetic groups grew in weight and length over the period analyzed, being influenced by the genetics of each species. O. bonariensis showed the best performance in growth and hybrid fish showed intermediate growth and estimated maximum body weight exceeding the pure species. In second experiment 25,806 potentially amplifiable microsatellite loci were identified in the species of pejerrey O. humensis. Of total loci, 167 were classified as high quality, with the ability to be highly variable in the population and with great potential for the analysis of genetic variability of this species. In general, it would be useful selective breeding, the aid of microsatellite markers, and formation of domesticated strains.