Avaliação in vitro da atividade de diferentes extratos de Origanum vulgare L. e Thymus vulgaris frente a microrganismos de importância veterinária

Abstract

The etnoveterinary has as one of their objectives to validate the several communities' knowledge in the use of medicines elaborated starting from medicinal plants, mainly in developing countries, that need alternatives economically viable seeking the good to be animal. With base in these objectives the study of the activity of the Origanum vulgare L. was accomplished and of Thymus vulgaris L., plants that you/they are used traditionally as seasoning, through three forms of different extraction for it drags of steam, maceration and decoction. Tests in vitro of essential oil, dye and aqueous extract were accomplished, front to microorganisms related to two illnesses the dermatofitosis and the external otititis. Inside of these illnesses they were selected three fungus that cause dermatofitosis (Microsporum canis, Trichophyton mentagrophytes and Microsporum gypseum) a yeast, the Malassezia pachydermatis, and two bacteria (Pseudomonas aeruginosa and Staphylococcus aureus) involved in the external otitis in dogs for us to be appraised front to the different extracts of the plants. The both microdiluition method was used in broth and the results were expressed in Minimal Inhibitory Concentrations (MIC-µl/ml) The essential oils were extracted through the modified Clevenger apparatus and the samples were submitted to the analysis by Gaseous Chromatography coupled to the Espectrometry of Masses (GC/EM) for their main known representatives' characterization as timol and the carvacrol. The essential oils presented lower MIC (1%), however, the methanol extract presented a better income for amount of plant and obtained extract. The extracts that presented better results were of the plant Origanum vulgare L.