Ocorrência e caracterização molecular e sorotipificação de Listeria monocytogenes e Listeria innocua isoladas em um frigorífico de suínos da Região das Missões – RS

Abstract

No abstracr.

Listeria monocytogenes is bacterium widely distributed in nature which presents high capacity to colonize surfaces and form biofilms in food processing plants. It distinguishes itself among the most important and dangerous microorganisms that cause food-borne diseases, such as listeriosis, a serious disease that causes abortion, neurological and gastrointestinal disorders, being the meat its main vehicle for transmission to humans. The aim of the present paper was to investigate the occurrence and molecularment characterization and sorotipificação of Listeria monocytogenes and Listeria innocua isolated in one swine frigorific from the Missions Region – RS. Listerias spp. was evaluated in water, cold chambers, carcasses, knives, faeces, hands and drains and the strains obtained went through serotyping and molecular typing through the analysis by random amplification of polymorphic DNA (RAPD) with UBC 155 e UBC 127 primers. The occurrence of L. monocytogenes was of 0,95% and of L. innocua, 18,1%, in 105 samples assayed, being the cold chambers the major point of isolation. Most of the L. innocua strains were not serotypable and, from those serotypable, the serovars 6a and 6b were prevalent. Only one L. monocytogenes strain was isolated, from a drain, and it belonged to serovar 4b. RAPD produced 15 genetic combined profiles. There is contamination by L. monocytogenes e L. inoccu in the swine frigorific, and this cross contamination in this plant is considered important, since some L. innocua strains persist in the local.