Impacto da suplementação de Vitamina C sobre níveis de peroxidação lipídica e glutationa reduzida em tecido hepático de camundongos com imunossupressão induzida por ciclofosfamida

Abstract

Cyclophosphamide is widely used in cancer treatment and as an immunosuppressant in transplant. This chemotherapy induces oxidative damage by increasing reactive oxygen species. Vitamin C is an essential nutrient important for the body because it has antioxidant activity. The present study aims to investigate the effects of vitamin C on levels of lipid peroxidation and reduced glutathione in liver tissue of mice immunosuppressed by cyclophosphamide. The study was performed in Swiss mice, female, 45 days old. The animals were divided into four groups of eight animals each. Groups: control group, vitamin C, cyclophosphamide group and treatment group (vitamin C and cyclophosphamide). The treatment lasted six days and on the seventh euthanasia of animals. The biochemical analysis of lipid peroxidation, and reduced glutathione were performed in liver tissue. The cyclophosphamide caused a significant increase (p <0.0001) in the levels of lipid peroxidation. No significant changes were observed in the groups treated with vitamin C and there was no interaction between vitamin C and cyclophosphamide. The cyclophosphamide did not alter levels of reduced glutathione. Vitamin C has reduced the level of reduced glutathione in the control, both in animals which received cyclophosphamide as the control and was no interaction between vitamin C and cyclophosphamide, or intensified chemotherapy the decrease of reduced glutathione caused by Vitamin C. The cyclophosphamide at a dose and time period used was able to induce oxidative damage verified by increased lipid peroxidation. The vitamin C in a dose of 50 mg / kg showed no potential to protect against oxidative damage caused by chemotherapy.